Pathogenesis of Eutypa lata in grapevine: identification of virulence factors and biochemical characterization of cordon dieback.
Identifieur interne : 000791 ( Main/Exploration ); précédent : 000790; suivant : 000792Pathogenesis of Eutypa lata in grapevine: identification of virulence factors and biochemical characterization of cordon dieback.
Auteurs : P E Rolshausen [États-Unis] ; L C Greve ; J M Labavitch ; N E Mahoney ; R J Molyneux ; W D GublerSource :
- Phytopathology [ 0031-949X ] ; 2008.
Descripteurs français
- KwdFr :
- Facteurs de virulence (métabolisme), Glucose (métabolisme), Glycosidases (métabolisme), Maladies des plantes (microbiologie), Oxidoreductases (métabolisme), Paroi cellulaire (métabolisme), Polyosides (métabolisme), Protéines fongiques (métabolisme), Virulence (MeSH), Vitis (microbiologie), Xylariales (enzymologie), Xylariales (métabolisme), Xylariales (pathogénicité), Xylose (métabolisme).
- MESH :
- enzymologie : Xylariales.
- microbiologie : Maladies des plantes, Vitis.
- métabolisme : Facteurs de virulence, Glucose, Glycosidases, Oxidoreductases, Paroi cellulaire, Polyosides, Protéines fongiques, Xylariales, Xylose.
- pathogénicité : Xylariales.
- Virulence.
English descriptors
- KwdEn :
- Cell Wall (metabolism), Fungal Proteins (metabolism), Glucose (metabolism), Glycoside Hydrolases (metabolism), Oxidoreductases (metabolism), Plant Diseases (microbiology), Polysaccharides (metabolism), Virulence (MeSH), Virulence Factors (metabolism), Vitis (microbiology), Xylariales (enzymology), Xylariales (metabolism), Xylariales (pathogenicity), Xylose (metabolism).
- MESH :
- chemical , metabolism : Fungal Proteins, Glucose, Glycoside Hydrolases, Oxidoreductases, Polysaccharides, Virulence Factors, Xylose.
- enzymology : Xylariales.
- metabolism : Cell Wall, Xylariales.
- microbiology : Plant Diseases, Vitis.
- pathogenicity : Xylariales.
- Virulence.
Abstract
Eutypa lata is a vascular pathogen of woody plants. In the present study we (i) determined which component(s) of the cell wall polymers were degraded in naturally infected grapevines and in artificially inoculated grape wood blocks; (ii) compared the pattern of wood decay in the tolerant grape cv. Merlot versus the susceptible cv. Cabernet Sauvignon; and (iii) identified secondary metabolites and hydrolytic enzymes expressed by E. lata during wood degradation. Biochemical analyses and a cytochemical study indicated that glucose-rich polymers were primary targets of E. lata. Structural glucose and xylose of the hemicellulose fraction of the plant cell wall and starch were depleted in infected woods identically in both cultivars. Moreover, the more tolerant cv. Merlot always had more lignin in the wood than the susceptible cv. Cabernet Sauvignon, indicating that this polymer may play a role in disease resistance. In vitro assays demonstrated the production by E. lata of oxidases, glycosidases and starch degrading enzymes. Phytotoxic secondary metabolites were also produced but our data suggest that they may bind to the wood. Finally, we demonstrated that free glucose in liquid cultures repressed primary but not secondary metabolism.
DOI: 10.1094/PHYTO-98-2-0222
PubMed: 18943199
Affiliations:
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Le document en format XML
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uniqKey="Mahoney N" first="N E" last="Mahoney">N E Mahoney</name></author><author><name sortKey="Molyneux, R J" sort="Molyneux, R J" uniqKey="Molyneux R" first="R J" last="Molyneux">R J Molyneux</name></author><author><name sortKey="Gubler, W D" sort="Gubler, W D" uniqKey="Gubler W" first="W D" last="Gubler">W D Gubler</name></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term><term>Fungal Proteins (metabolism)</term><term>Glucose (metabolism)</term><term>Glycoside Hydrolases (metabolism)</term><term>Oxidoreductases (metabolism)</term><term>Plant Diseases (microbiology)</term><term>Polysaccharides (metabolism)</term><term>Virulence (MeSH)</term><term>Virulence Factors (metabolism)</term><term>Vitis (microbiology)</term><term>Xylariales (enzymology)</term><term>Xylariales (metabolism)</term><term>Xylariales (pathogenicity)</term><term>Xylose (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Facteurs de virulence (métabolisme)</term><term>Glucose (métabolisme)</term><term>Glycosidases (métabolisme)</term><term>Maladies des plantes (microbiologie)</term><term>Oxidoreductases (métabolisme)</term><term>Paroi cellulaire (métabolisme)</term><term>Polyosides (métabolisme)</term><term>Protéines fongiques (métabolisme)</term><term>Virulence (MeSH)</term><term>Vitis (microbiologie)</term><term>Xylariales (enzymologie)</term><term>Xylariales (métabolisme)</term><term>Xylariales (pathogénicité)</term><term>Xylose (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Glucose</term><term>Glycoside Hydrolases</term><term>Oxidoreductases</term><term>Polysaccharides</term><term>Virulence Factors</term><term>Xylose</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Xylariales</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Xylariales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Xylariales</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de virulence</term><term>Glucose</term><term>Glycosidases</term><term>Oxidoreductases</term><term>Paroi cellulaire</term><term>Polyosides</term><term>Protéines 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In the present study we (i) determined which component(s) of the cell wall polymers were degraded in naturally infected grapevines and in artificially inoculated grape wood blocks; (ii) compared the pattern of wood decay in the tolerant grape cv. Merlot versus the susceptible cv. Cabernet Sauvignon; and (iii) identified secondary metabolites and hydrolytic enzymes expressed by E. lata during wood degradation. Biochemical analyses and a cytochemical study indicated that glucose-rich polymers were primary targets of E. lata. Structural glucose and xylose of the hemicellulose fraction of the plant cell wall and starch were depleted in infected woods identically in both cultivars. Moreover, the more tolerant cv. Merlot always had more lignin in the wood than the susceptible cv. Cabernet Sauvignon, indicating that this polymer may play a role in disease resistance. In vitro assays demonstrated the production by E. lata of oxidases, glycosidases and starch degrading enzymes. Phytotoxic secondary metabolites were also produced but our data suggest that they may bind to the wood. Finally, we demonstrated that free glucose in liquid cultures repressed primary but not secondary metabolism.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18943199</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>19</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>98</Volume><Issue>2</Issue><PubDate><Year>2008</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Pathogenesis of Eutypa lata in grapevine: identification of virulence factors and biochemical characterization of cordon dieback.</ArticleTitle><Pagination><MedlinePgn>222-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-98-2-0222</ELocationID><Abstract><AbstractText>Eutypa lata is a vascular pathogen of woody plants. In the present study we (i) determined which component(s) of the cell wall polymers were degraded in naturally infected grapevines and in artificially inoculated grape wood blocks; (ii) compared the pattern of wood decay in the tolerant grape cv. Merlot versus the susceptible cv. Cabernet Sauvignon; and (iii) identified secondary metabolites and hydrolytic enzymes expressed by E. lata during wood degradation. Biochemical analyses and a cytochemical study indicated that glucose-rich polymers were primary targets of E. lata. Structural glucose and xylose of the hemicellulose fraction of the plant cell wall and starch were depleted in infected woods identically in both cultivars. Moreover, the more tolerant cv. Merlot always had more lignin in the wood than the susceptible cv. Cabernet Sauvignon, indicating that this polymer may play a role in disease resistance. In vitro assays demonstrated the production by E. lata of oxidases, glycosidases and starch degrading enzymes. Phytotoxic secondary metabolites were also produced but our data suggest that they may bind to the wood. Finally, we demonstrated that free glucose in liquid cultures repressed primary but not secondary metabolism.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rolshausen</LastName><ForeName>P E</ForeName><Initials>PE</Initials><AffiliationInfo><Affiliation>University of California, Department of Plant Pathology, Davis, CA 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Greve</LastName><ForeName>L C</ForeName><Initials>LC</Initials></Author><Author ValidYN="Y"><LastName>Labavitch</LastName><ForeName>J M</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Mahoney</LastName><ForeName>N E</ForeName><Initials>NE</Initials></Author><Author ValidYN="Y"><LastName>Molyneux</LastName><ForeName>R J</ForeName><Initials>RJ</Initials></Author><Author ValidYN="Y"><LastName>Gubler</LastName><ForeName>W D</ForeName><Initials>WD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037521">Virulence Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>8024-50-8</RegistryNumber><NameOfSubstance 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PubStatus="medline"><Year>2009</Year><Month>3</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18943199</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-98-2-0222</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Greve, L C" sort="Greve, L C" uniqKey="Greve L" first="L C" last="Greve">L C Greve</name><name sortKey="Gubler, W D" sort="Gubler, W D" uniqKey="Gubler W" first="W D" last="Gubler">W D Gubler</name><name sortKey="Labavitch, J M" sort="Labavitch, J M" uniqKey="Labavitch J" first="J M" last="Labavitch">J M Labavitch</name><name sortKey="Mahoney, N E" sort="Mahoney, N E" uniqKey="Mahoney N" first="N E" last="Mahoney">N E Mahoney</name><name sortKey="Molyneux, R J" sort="Molyneux, R J" uniqKey="Molyneux R" first="R J" last="Molyneux">R J Molyneux</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Rolshausen, P E" sort="Rolshausen, P E" uniqKey="Rolshausen P" first="P E" last="Rolshausen">P E Rolshausen</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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